cell lines spodoptera frugiperda cells sf9 expression systems (Expression Systems Inc)
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cell lines spodoptera frugiperda cells sf9 expression systems
Cell Lines Spodoptera Frugiperda Cells Sf9 Expression Systems, supplied by Expression Systems Inc, used in various techniques. Bioz Stars score: 99/100, based on 4762 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cell+lines+spodoptera+frugiperda+sf9+cells+expression+systems/Sf9+Cells/pm41747717-194-35-41
Average 99 stars, based on 4762 article reviews
Cell Lines Spodoptera Frugiperda Cells Sf9 Expression Systems, supplied by Expression Systems Inc, used in various techniques. Bioz Stars score: 99/100, based on 4762 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cell+lines+spodoptera+frugiperda+sf9+cells+expression+systems/Sf9+Cells/pm41747717-194-35-41
Average 99 stars, based on 4762 article reviews
cell lines spodoptera frugiperda cells sf9 expression systems - by Bioz Stars,
2026-09
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Recombinant:Article Title: High-affinity agonists reveal recognition motifs for the MRGPRD GPCR. Article Snippet: Report Article Title: Signaling snapshots of a serotonin receptor activated by the prototypical psychedelic LSD. Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies gp64-PE antibody Expression Systems Cat#97-201; RRID:AB_2922960 Chemicals, peptides, and recombinant proteins AEBSF GoldBio Cat#A-540-5 Leupeptin Sigma Cat#L2884 E-64 AG Scientific Cat# E-2030-25MG Aprotinin GoldBio Cat#A-655-100 n-dodecyl-beta-D-Maltopyranoside (DDM) Anatrace Cat#D310 Lauryl maltose neopentyl glycol (LMNG) Anatrace Cat#NG310 Glyco-Diosgenin (GDN) Anatrace Cat#GDN101 Cholesteryl Hemisuccinate Tris Salt Anatrace Cat#CH210 25GM His-tagged PreScission protease GenScript Cat#Z0392-500 Tris HCl VWR Cat#0497 HEPES Fisher Scientific Cat#BP310 Glycerol Fisher Scientific Cat#BP229 Imidazole Sigma Cat#I0250 Sodium Chloride Fisher Cat#BP358 Potassium Chloride Sigma Cat#793590 Magnesium Chloride Sigma Cat#M0250 TALON IMAC resin Clontech Cat#635507 Coelenterazine-h Promega Cat#S2011 Poly-L-lysine Sigma Cat#P2636 Penicillin/Streptomycin Invitrogen Cat#15140-122 Sf-900 II SFM Invitrogen Cat#10902096 ESF921 Expression Systems Cat#96-001-01 DMEM Sigma Cat# D6429-6X50 USDA FBS Omega Scientific Cat#2463 FB-01 Dialyzed FBS Omega Scientific Cat#2459 FB-03 10xHBSS Invitrogen Cat#14065-056 BSA, Free Fatty Acid Akron Cat#AK8909 Ascorbic Acid Sigma Cat#A5960 Cellfectin II Reagent Invitrogen Cat#10362-100 TransIT-2020 Mirus Cat#MIR5400 Critical commercial assays Bac-to-Bac Baculovirus Expression system Invitrogen Cat#A11100 pcDNA 3.1 Thermo Fisher Cat#V79020 Deposited data HTR2B/b-arrestin-1 This paper PDB: 7SRS HTR2B-LSD/miniGq This paper PDB: 7SRR HTR2B-LSD This paper PDB: 7SRQ Experimental models: Article Title: Structural basis for ion selectivity in potassium-selective channelrhodopsins. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and virus strains One Shot Stbl3 Chemically Competent E. coli Thermo Fisher Scientific Cat# C737303 E. coli BL21 (DE3) Thermo Fisher Scientific Cat# EC0114 E. coli C41 (DE3) Sigma-Aldrich Cat# CMC0017 E. coli Mach1 Thermo Fisher Scientific Cat# C862003 E. coli DH10Bac Thermo Fisher Scientific Cat# 10361012 Adeno-Associated Virus Coat Protein 2/8 Constructed in the Deisseroth lab; packaged at the Stanford Gene Vector and Virus Core (GVVC) AAV8 AAV8-CaMKIIa-HcKCR1 (WT)-EYFP Constructed in the Deisseroth lab; packaged at the GVVC N/A AAV8-CaMKIIa-HcKCR1 (H225F)-EYFP Constructed in the Deisseroth lab; packaged at the GVVC N/A AAV8-CaMKIIa-HcKCR2 (WT)-EYFP Constructed in the Deisseroth lab; packaged at the GVVC N/A AAV8-CaMKIIa-HcKCR2 (H225F)-EYFP Constructed in the Deisseroth lab; packaged at the GVVC N/A AAV8-CaMKIIa-XCaMP-R Constructed in the Deisseroth lab; packaged at the GVVC N/A AAV8-CaMKIIa-sRGECO Constructed in the Deisseroth lab; packaged at the GVVC N/A AAV8-CaMKIIa-NpHR3.3-mCherry Constructed in the Deisseroth lab; packaged at the GVVC N/A AAV8-CaMKIIa-GCaMP6m Constructed in the Deisseroth lab; packaged at the GVVC N/A Chemicals, peptides, and recombinant proteins APV Tocris Cat# 0106 CNQX Tocris Cat# 0190 Tetrodotoxin Tocris Cat# 1078 Fluorodeoxyuridine Sigma Cat# F0503 Lipofectamine 2000 Thermo Fisher Scientific Cat# 11668027 FuGENE Promega Cat# E2311 Benzamidine Nacalai tesque Cat# M9G4533 Leupeptin Peptide Institute Cat# 4041 n-Dodecyl-b-D-maltoside (DDM) EMD Millipore Cat# 324355 n-Dodecyl-b-D-maltoside (DDM) EMD Millipore Cat# D97002-C Ni-NTA Superflow QIAGEN Cat# 30430 Superdex 200 Increase 10/300 GL Cytiva Cat# 28990944 TSKgel3000SWXL column TOSOH bioscience Cat# 0008541 All-trans-retinal (ATR) Sigma-Aldrich Cat# R2500 StockOptions pH buffer kit Hampton Research Cat# HR2-241 ESF 921 Insect Cell Culture Medium, Protein Free Expression systems Cat# 96-001-01 Monolith His-Tag Labeling Kit RED-tris-NTA 2ND Generation NanoTemper Technologies Cat# MO-L018 Deposited data HcKCR1 – atomic model From this paper PDB: 8H86 (Continued on next page) Cell 186, 4325–4344.e1–e13, September 28, 2023 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER HcKCR2 – atomic model From this paper PDB: 8H87 HcKCR1 H225F mutant– atomic model From this paper PDB: 8IU0 HcKCR1 – Cryo-EM map From this paper EMDB-34530 HcKCR2 – Cryo-EM map From this paper EMDB-34531 HcKCR1 H225F mutant – Cryo-EM map From this paper EMDB-35713 HcKCR1 – Cryo-EM movie frames From this paper EMPIAR-11558 HcKCR2 – Cryo-EM movie frames From this paper EMPIAR-11558 HcKCR1 H225F mutant – Cryo-EM movie frames From this paper EMPIAR-11558 Experimental models: Article Title: Structures of the entire human opioid receptor family. Article Snippet: Article Structures of the entire human opioid receptor family Article Title: Structural and signaling mechanisms of TAAR1 enabled preferential agonist design. Article Snippet: Article Structural and signaling mechanisms of TAAR1 enabled preferential agonist design Article Title: Structural basis for ligand recognition and activation of the prostanoid receptors. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Monoclonal ANTI-FLAG M2-FITC antibody produced in mouse Sigma Aldrich Cat# F4049; RRID:AB_439701 Baculovirus Envelope gp64 Monoclonal Antibody (AcV1), PE Invitrogen Cat# 12-6991-82; RRID:AB_1633369 Bacterial and virus strains Trans5a Competent Cells Trans Cat# CD201 Chemicals, peptides, and recombinant proteins Prostaglandin F2a (PGF2a) Cayman Cat# 16010 Latanoprost acid Sigma Aldrich Cat# L1292 Cloprostenol Cayman Cat# 16764 U46619 Sigma Aldrich Cat# D-8174 Lauryl maltose neopentyl glycol (LMNG) Anatrace Cat# NG310 Cholesteryl hemisuccinate tris salt (CHS) Sigma Aldrich Cat# C6013 Glyco-diosgenin (GDN) Anatrace Cat# GDN101 Leupeptin hydrochloride Sigma Aldrich Cat# L9783 Benzamidine Sigma Aldrich Cat# 12072 Tris (2-carboxyethyl) phosphine hydrochloride Sigma Aldrich Cat# 75259 Apyrase New England Biolabs Cat# M0398L ANTI-FLAG M1 Agarose Affinity Gel Sigma Aldrich Cat# A4596 Nickel Sepharose resin GE healthcare Cat# 17526801 FLAG peptide Sangon Biotech Custom Synthesis FuGENE Transfection Reagent Promega Cat# E2311 Lipofectamine 3000 Transfection Kit Invitrogen Cat# L3000015 ESF921 culture medium Expression systems Cat# 96-001-01 Dulbecco’s Modified Eagle Medium (DMEM) gibco Cat# 8122600 Fetal bovine serum (FBS) gibco Cat# 10099141c Hanks’ Balanced Salt Solution (HBSS) gibco Cat# 2276630 bovine serum albumin (BSA) Sigma Aldrich Cat# A1933 Coelenterazine 400a Maokangbio Cat# MX4610 Trypsin EDTA Solution A VivaCell Biosciences Cat# C3530-0100 PEI MAX -Transfection Grade Linear Polyethylenimine Hydrochloride Polysciences Cat# 24765-1 Phosphate buffered solution (PBS) Solarbio Cat# P1003 Critical commercial assays Bac-to-Bac Baculovirus Expression System Invitrogen Cat# A11098 IP-One Gq kit Cisbio Cat# 621PAPEB Deposited data FP-Cloprostenol-Gq coordinates This paper PDB: 8XJK FP-PGF2a-Gq coordinates This paper PDB: 8XJL FP-Latanoprost acid-Gq This paper PDB: 8XJM TP-Cloprostenol-Gq This paper PDB: 8XJN TP-U46619-Gq This paper PDB: 8XJO (Continued on next page) Cell Reports 43, 113893, March 26, 2024 15 .. REAGENT or RESOURCE SOURCE IDENTIFIER FP-Cloprostenol-Gq EM map This paper EMDB: EMD-38399 FP-PGF2a-Gq EM map This paper EMDB: EMD-38400 FP-Latanoprost acid-Gq EM map This paper EMDB: EMD-38401 TP-Cloprostenol-Gq EM map This paper EMDB: EMD-38402 TP-U46619-Gq EM map This paper EMDB: EMD-38403 Experimental models: Expressing:Article Title: High-affinity agonists reveal recognition motifs for the MRGPRD GPCR. Article Snippet: Report Software:Article Title: High-affinity agonists reveal recognition motifs for the MRGPRD GPCR. Article Snippet: Report Article Title: Structural basis for ion selectivity in potassium-selective channelrhodopsins. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and virus strains One Shot Stbl3 Chemically Competent E. coli Thermo Fisher Scientific Cat# C737303 E. coli BL21 (DE3) Thermo Fisher Scientific Cat# EC0114 E. coli C41 (DE3) Sigma-Aldrich Cat# CMC0017 E. coli Mach1 Thermo Fisher Scientific Cat# C862003 E. coli DH10Bac Thermo Fisher Scientific Cat# 10361012 Adeno-Associated Virus Coat Protein 2/8 Constructed in the Deisseroth lab; packaged at the Stanford Gene Vector and Virus Core (GVVC) AAV8 AAV8-CaMKIIa-HcKCR1 (WT)-EYFP Constructed in the Deisseroth lab; packaged at the GVVC N/A AAV8-CaMKIIa-HcKCR1 (H225F)-EYFP Constructed in the Deisseroth lab; packaged at the GVVC N/A AAV8-CaMKIIa-HcKCR2 (WT)-EYFP Constructed in the Deisseroth lab; packaged at the GVVC N/A AAV8-CaMKIIa-HcKCR2 (H225F)-EYFP Constructed in the Deisseroth lab; packaged at the GVVC N/A AAV8-CaMKIIa-XCaMP-R Constructed in the Deisseroth lab; packaged at the GVVC N/A AAV8-CaMKIIa-sRGECO Constructed in the Deisseroth lab; packaged at the GVVC N/A AAV8-CaMKIIa-NpHR3.3-mCherry Constructed in the Deisseroth lab; packaged at the GVVC N/A AAV8-CaMKIIa-GCaMP6m Constructed in the Deisseroth lab; packaged at the GVVC N/A Chemicals, peptides, and recombinant proteins APV Tocris Cat# 0106 CNQX Tocris Cat# 0190 Tetrodotoxin Tocris Cat# 1078 Fluorodeoxyuridine Sigma Cat# F0503 Lipofectamine 2000 Thermo Fisher Scientific Cat# 11668027 FuGENE Promega Cat# E2311 Benzamidine Nacalai tesque Cat# M9G4533 Leupeptin Peptide Institute Cat# 4041 n-Dodecyl-b-D-maltoside (DDM) EMD Millipore Cat# 324355 n-Dodecyl-b-D-maltoside (DDM) EMD Millipore Cat# D97002-C Ni-NTA Superflow QIAGEN Cat# 30430 Superdex 200 Increase 10/300 GL Cytiva Cat# 28990944 TSKgel3000SWXL column TOSOH bioscience Cat# 0008541 All-trans-retinal (ATR) Sigma-Aldrich Cat# R2500 StockOptions pH buffer kit Hampton Research Cat# HR2-241 ESF 921 Insect Cell Culture Medium, Protein Free Expression systems Cat# 96-001-01 Monolith His-Tag Labeling Kit RED-tris-NTA 2ND Generation NanoTemper Technologies Cat# MO-L018 Deposited data HcKCR1 – atomic model From this paper PDB: 8H86 (Continued on next page) Cell 186, 4325–4344.e1–e13, September 28, 2023 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER HcKCR2 – atomic model From this paper PDB: 8H87 HcKCR1 H225F mutant– atomic model From this paper PDB: 8IU0 HcKCR1 – Cryo-EM map From this paper EMDB-34530 HcKCR2 – Cryo-EM map From this paper EMDB-34531 HcKCR1 H225F mutant – Cryo-EM map From this paper EMDB-35713 HcKCR1 – Cryo-EM movie frames From this paper EMPIAR-11558 HcKCR2 – Cryo-EM movie frames From this paper EMPIAR-11558 HcKCR1 H225F mutant – Cryo-EM movie frames From this paper EMPIAR-11558 Experimental models: Article Title: Structural and signaling mechanisms of TAAR1 enabled preferential agonist design. Article Snippet: Article Structural and signaling mechanisms of TAAR1 enabled preferential agonist design Article Title: Structural basis for ligand recognition and activation of the prostanoid receptors. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Monoclonal ANTI-FLAG M2-FITC antibody produced in mouse Sigma Aldrich Cat# F4049; RRID:AB_439701 Baculovirus Envelope gp64 Monoclonal Antibody (AcV1), PE Invitrogen Cat# 12-6991-82; RRID:AB_1633369 Bacterial and virus strains Trans5a Competent Cells Trans Cat# CD201 Chemicals, peptides, and recombinant proteins Prostaglandin F2a (PGF2a) Cayman Cat# 16010 Latanoprost acid Sigma Aldrich Cat# L1292 Cloprostenol Cayman Cat# 16764 U46619 Sigma Aldrich Cat# D-8174 Lauryl maltose neopentyl glycol (LMNG) Anatrace Cat# NG310 Cholesteryl hemisuccinate tris salt (CHS) Sigma Aldrich Cat# C6013 Glyco-diosgenin (GDN) Anatrace Cat# GDN101 Leupeptin hydrochloride Sigma Aldrich Cat# L9783 Benzamidine Sigma Aldrich Cat# 12072 Tris (2-carboxyethyl) phosphine hydrochloride Sigma Aldrich Cat# 75259 Apyrase New England Biolabs Cat# M0398L ANTI-FLAG M1 Agarose Affinity Gel Sigma Aldrich Cat# A4596 Nickel Sepharose resin GE healthcare Cat# 17526801 FLAG peptide Sangon Biotech Custom Synthesis FuGENE Transfection Reagent Promega Cat# E2311 Lipofectamine 3000 Transfection Kit Invitrogen Cat# L3000015 ESF921 culture medium Expression systems Cat# 96-001-01 Dulbecco’s Modified Eagle Medium (DMEM) gibco Cat# 8122600 Fetal bovine serum (FBS) gibco Cat# 10099141c Hanks’ Balanced Salt Solution (HBSS) gibco Cat# 2276630 bovine serum albumin (BSA) Sigma Aldrich Cat# A1933 Coelenterazine 400a Maokangbio Cat# MX4610 Trypsin EDTA Solution A VivaCell Biosciences Cat# C3530-0100 PEI MAX -Transfection Grade Linear Polyethylenimine Hydrochloride Polysciences Cat# 24765-1 Phosphate buffered solution (PBS) Solarbio Cat# P1003 Critical commercial assays Bac-to-Bac Baculovirus Expression System Invitrogen Cat# A11098 IP-One Gq kit Cisbio Cat# 621PAPEB Deposited data FP-Cloprostenol-Gq coordinates This paper PDB: 8XJK FP-PGF2a-Gq coordinates This paper PDB: 8XJL FP-Latanoprost acid-Gq This paper PDB: 8XJM TP-Cloprostenol-Gq This paper PDB: 8XJN TP-U46619-Gq This paper PDB: 8XJO (Continued on next page) Cell Reports 43, 113893, March 26, 2024 15 .. REAGENT or RESOURCE SOURCE IDENTIFIER FP-Cloprostenol-Gq EM map This paper EMDB: EMD-38399 FP-PGF2a-Gq EM map This paper EMDB: EMD-38400 FP-Latanoprost acid-Gq EM map This paper EMDB: EMD-38401 TP-Cloprostenol-Gq EM map This paper EMDB: EMD-38402 TP-U46619-Gq EM map This paper EMDB: EMD-38403 Experimental models: Chromatography:Article Title: High-affinity agonists reveal recognition motifs for the MRGPRD GPCR. Article Snippet: Report Molecular Weight:Article Title: High-affinity agonists reveal recognition motifs for the MRGPRD GPCR. Article Snippet: Report Article Title: Structural and signaling mechanisms of TAAR1 enabled preferential agonist design. Article Snippet: Article Structural and signaling mechanisms of TAAR1 enabled preferential agonist design Electron Microscopy:Article Title: High-affinity agonists reveal recognition motifs for the MRGPRD GPCR. Article Snippet: Report Mutagenesis:Article Title: Structural basis for ion selectivity in potassium-selective channelrhodopsins. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and virus strains One Shot Stbl3 Chemically Competent E. coli Thermo Fisher Scientific Cat# C737303 E. coli BL21 (DE3) Thermo Fisher Scientific Cat# EC0114 E. coli C41 (DE3) Sigma-Aldrich Cat# CMC0017 E. coli Mach1 Thermo Fisher Scientific Cat# C862003 E. coli DH10Bac Thermo Fisher Scientific Cat# 10361012 Adeno-Associated Virus Coat Protein 2/8 Constructed in the Deisseroth lab; packaged at the Stanford Gene Vector and Virus Core (GVVC) AAV8 AAV8-CaMKIIa-HcKCR1 (WT)-EYFP Constructed in the Deisseroth lab; packaged at the GVVC N/A AAV8-CaMKIIa-HcKCR1 (H225F)-EYFP Constructed in the Deisseroth lab; packaged at the GVVC N/A AAV8-CaMKIIa-HcKCR2 (WT)-EYFP Constructed in the Deisseroth lab; packaged at the GVVC N/A AAV8-CaMKIIa-HcKCR2 (H225F)-EYFP Constructed in the Deisseroth lab; packaged at the GVVC N/A AAV8-CaMKIIa-XCaMP-R Constructed in the Deisseroth lab; packaged at the GVVC N/A AAV8-CaMKIIa-sRGECO Constructed in the Deisseroth lab; packaged at the GVVC N/A AAV8-CaMKIIa-NpHR3.3-mCherry Constructed in the Deisseroth lab; packaged at the GVVC N/A AAV8-CaMKIIa-GCaMP6m Constructed in the Deisseroth lab; packaged at the GVVC N/A Chemicals, peptides, and recombinant proteins APV Tocris Cat# 0106 CNQX Tocris Cat# 0190 Tetrodotoxin Tocris Cat# 1078 Fluorodeoxyuridine Sigma Cat# F0503 Lipofectamine 2000 Thermo Fisher Scientific Cat# 11668027 FuGENE Promega Cat# E2311 Benzamidine Nacalai tesque Cat# M9G4533 Leupeptin Peptide Institute Cat# 4041 n-Dodecyl-b-D-maltoside (DDM) EMD Millipore Cat# 324355 n-Dodecyl-b-D-maltoside (DDM) EMD Millipore Cat# D97002-C Ni-NTA Superflow QIAGEN Cat# 30430 Superdex 200 Increase 10/300 GL Cytiva Cat# 28990944 TSKgel3000SWXL column TOSOH bioscience Cat# 0008541 All-trans-retinal (ATR) Sigma-Aldrich Cat# R2500 StockOptions pH buffer kit Hampton Research Cat# HR2-241 ESF 921 Insect Cell Culture Medium, Protein Free Expression systems Cat# 96-001-01 Monolith His-Tag Labeling Kit RED-tris-NTA 2ND Generation NanoTemper Technologies Cat# MO-L018 Deposited data HcKCR1 – atomic model From this paper PDB: 8H86 (Continued on next page) Cell 186, 4325–4344.e1–e13, September 28, 2023 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER HcKCR2 – atomic model From this paper PDB: 8H87 HcKCR1 H225F mutant– atomic model From this paper PDB: 8IU0 HcKCR1 – Cryo-EM map From this paper EMDB-34530 HcKCR2 – Cryo-EM map From this paper EMDB-34531 HcKCR1 H225F mutant – Cryo-EM map From this paper EMDB-35713 HcKCR1 – Cryo-EM movie frames From this paper EMPIAR-11558 HcKCR2 – Cryo-EM movie frames From this paper EMPIAR-11558 HcKCR1 H225F mutant – Cryo-EM movie frames From this paper EMPIAR-11558 Experimental models: Cryo-EM Sample Prep:Article Title: Structural basis for ion selectivity in potassium-selective channelrhodopsins. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and virus strains One Shot Stbl3 Chemically Competent E. coli Thermo Fisher Scientific Cat# C737303 E. coli BL21 (DE3) Thermo Fisher Scientific Cat# EC0114 E. coli C41 (DE3) Sigma-Aldrich Cat# CMC0017 E. coli Mach1 Thermo Fisher Scientific Cat# C862003 E. coli DH10Bac Thermo Fisher Scientific Cat# 10361012 Adeno-Associated Virus Coat Protein 2/8 Constructed in the Deisseroth lab; packaged at the Stanford Gene Vector and Virus Core (GVVC) AAV8 AAV8-CaMKIIa-HcKCR1 (WT)-EYFP Constructed in the Deisseroth lab; packaged at the GVVC N/A AAV8-CaMKIIa-HcKCR1 (H225F)-EYFP Constructed in the Deisseroth lab; packaged at the GVVC N/A AAV8-CaMKIIa-HcKCR2 (WT)-EYFP Constructed in the Deisseroth lab; packaged at the GVVC N/A AAV8-CaMKIIa-HcKCR2 (H225F)-EYFP Constructed in the Deisseroth lab; packaged at the GVVC N/A AAV8-CaMKIIa-XCaMP-R Constructed in the Deisseroth lab; packaged at the GVVC N/A AAV8-CaMKIIa-sRGECO Constructed in the Deisseroth lab; packaged at the GVVC N/A AAV8-CaMKIIa-NpHR3.3-mCherry Constructed in the Deisseroth lab; packaged at the GVVC N/A AAV8-CaMKIIa-GCaMP6m Constructed in the Deisseroth lab; packaged at the GVVC N/A Chemicals, peptides, and recombinant proteins APV Tocris Cat# 0106 CNQX Tocris Cat# 0190 Tetrodotoxin Tocris Cat# 1078 Fluorodeoxyuridine Sigma Cat# F0503 Lipofectamine 2000 Thermo Fisher Scientific Cat# 11668027 FuGENE Promega Cat# E2311 Benzamidine Nacalai tesque Cat# M9G4533 Leupeptin Peptide Institute Cat# 4041 n-Dodecyl-b-D-maltoside (DDM) EMD Millipore Cat# 324355 n-Dodecyl-b-D-maltoside (DDM) EMD Millipore Cat# D97002-C Ni-NTA Superflow QIAGEN Cat# 30430 Superdex 200 Increase 10/300 GL Cytiva Cat# 28990944 TSKgel3000SWXL column TOSOH bioscience Cat# 0008541 All-trans-retinal (ATR) Sigma-Aldrich Cat# R2500 StockOptions pH buffer kit Hampton Research Cat# HR2-241 ESF 921 Insect Cell Culture Medium, Protein Free Expression systems Cat# 96-001-01 Monolith His-Tag Labeling Kit RED-tris-NTA 2ND Generation NanoTemper Technologies Cat# MO-L018 Deposited data HcKCR1 – atomic model From this paper PDB: 8H86 (Continued on next page) Cell 186, 4325–4344.e1–e13, September 28, 2023 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER HcKCR2 – atomic model From this paper PDB: 8H87 HcKCR1 H225F mutant– atomic model From this paper PDB: 8IU0 HcKCR1 – Cryo-EM map From this paper EMDB-34530 HcKCR2 – Cryo-EM map From this paper EMDB-34531 HcKCR1 H225F mutant – Cryo-EM map From this paper EMDB-35713 HcKCR1 – Cryo-EM movie frames From this paper EMPIAR-11558 HcKCR2 – Cryo-EM movie frames From this paper EMPIAR-11558 HcKCR1 H225F mutant – Cryo-EM movie frames From this paper EMPIAR-11558 Experimental models: Cell Culture:Article Title: Structural basis for ion selectivity in potassium-selective channelrhodopsins. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and virus strains One Shot Stbl3 Chemically Competent E. coli Thermo Fisher Scientific Cat# C737303 E. coli BL21 (DE3) Thermo Fisher Scientific Cat# EC0114 E. coli C41 (DE3) Sigma-Aldrich Cat# CMC0017 E. coli Mach1 Thermo Fisher Scientific Cat# C862003 E. coli DH10Bac Thermo Fisher Scientific Cat# 10361012 Adeno-Associated Virus Coat Protein 2/8 Constructed in the Deisseroth lab; packaged at the Stanford Gene Vector and Virus Core (GVVC) AAV8 AAV8-CaMKIIa-HcKCR1 (WT)-EYFP Constructed in the Deisseroth lab; packaged at the GVVC N/A AAV8-CaMKIIa-HcKCR1 (H225F)-EYFP Constructed in the Deisseroth lab; packaged at the GVVC N/A AAV8-CaMKIIa-HcKCR2 (WT)-EYFP Constructed in the Deisseroth lab; packaged at the GVVC N/A AAV8-CaMKIIa-HcKCR2 (H225F)-EYFP Constructed in the Deisseroth lab; packaged at the GVVC N/A AAV8-CaMKIIa-XCaMP-R Constructed in the Deisseroth lab; packaged at the GVVC N/A AAV8-CaMKIIa-sRGECO Constructed in the Deisseroth lab; packaged at the GVVC N/A AAV8-CaMKIIa-NpHR3.3-mCherry Constructed in the Deisseroth lab; packaged at the GVVC N/A AAV8-CaMKIIa-GCaMP6m Constructed in the Deisseroth lab; packaged at the GVVC N/A Chemicals, peptides, and recombinant proteins APV Tocris Cat# 0106 CNQX Tocris Cat# 0190 Tetrodotoxin Tocris Cat# 1078 Fluorodeoxyuridine Sigma Cat# F0503 Lipofectamine 2000 Thermo Fisher Scientific Cat# 11668027 FuGENE Promega Cat# E2311 Benzamidine Nacalai tesque Cat# M9G4533 Leupeptin Peptide Institute Cat# 4041 n-Dodecyl-b-D-maltoside (DDM) EMD Millipore Cat# 324355 n-Dodecyl-b-D-maltoside (DDM) EMD Millipore Cat# D97002-C Ni-NTA Superflow QIAGEN Cat# 30430 Superdex 200 Increase 10/300 GL Cytiva Cat# 28990944 TSKgel3000SWXL column TOSOH bioscience Cat# 0008541 All-trans-retinal (ATR) Sigma-Aldrich Cat# R2500 StockOptions pH buffer kit Hampton Research Cat# HR2-241 ESF 921 Insect Cell Culture Medium, Protein Free Expression systems Cat# 96-001-01 Monolith His-Tag Labeling Kit RED-tris-NTA 2ND Generation NanoTemper Technologies Cat# MO-L018 Deposited data HcKCR1 – atomic model From this paper PDB: 8H86 (Continued on next page) Cell 186, 4325–4344.e1–e13, September 28, 2023 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER HcKCR2 – atomic model From this paper PDB: 8H87 HcKCR1 H225F mutant– atomic model From this paper PDB: 8IU0 HcKCR1 – Cryo-EM map From this paper EMDB-34530 HcKCR2 – Cryo-EM map From this paper EMDB-34531 HcKCR1 H225F mutant – Cryo-EM map From this paper EMDB-35713 HcKCR1 – Cryo-EM movie frames From this paper EMPIAR-11558 HcKCR2 – Cryo-EM movie frames From this paper EMPIAR-11558 HcKCR1 H225F mutant – Cryo-EM movie frames From this paper EMPIAR-11558 Experimental models: other:Article Title: Cryo-EM structures of Clostridium perfringens enterotoxin bound to its human receptor, claudin-4. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Synthetic antibody Fab (sFab) COP-1 Erramilli et al.47 N/A Bacterial and virus strains E. coli XL1-Blue Agilent Technologies Cat# 200236 E. coli BL21-Gold Agilent Technologies Cat# 200131 Chemicals, peptides, and Lysis:Article Title: Structural and signaling mechanisms of TAAR1 enabled preferential agonist design. Article Snippet: Article Structural and signaling mechanisms of TAAR1 enabled preferential agonist design |